| 摘要: |
| ［摘要］ 目的 研究神经生长因子(NGF)对子宫腺肌病患者离体培养在位子宫内膜基质细胞雌激素受体α表达的影响。方法 用10 ng/ml、50 ng/ml和100 ng/ml的神经生长因子分别对体外原代培养人子宫内膜细胞进行干预，用MTT法测定内膜基质细胞生长的增殖情况，用Westen-blotting测定50 ng/ml NGF对病例组基质细胞雌激素受体α表达的变化。结果 50 ng/ml的NGF可促进子宫内膜基质细胞增殖，细胞存活率为82.61%,比其他浓度NGF显著升高(P<0.05);50 ng/ml NGF对病例组和对照组不同细胞雌激素受体表达有影响，病例组腺上皮细胞和基质细胞总体均数差异均具有统计学意义(P<0.01)。结论 50 ng/ml的NGF可促进子宫腺肌病在位内膜基质细胞增殖及腺上皮细胞和基质细胞雌激素受体α表达。 |
| 关键词: 神经生长因子 子宫腺肌病 雌激素受体 |
| DOI：10.3969/j.issn.1674-3806.2011.10.09 |
| 分类号:R 711.74 |
| 基金项目: |
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| Effect of nerve growth factor on stromal cell proliferation and estrogen receptor α expression of eutopic endometrial cells of adenomyosis uterine |
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XIE An-tuan
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Department of Obstetrics and Gynecology,the Lianping County Hospital,Guangdong 517100,China
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| Abstract: |
| ［Abstract］ Objective To investigate the effect of nerve growth factor on stromal cell proliferation and estrogen receptor α(ERα) expression of eutopic endometrial cells of adenomyosis uterine.Methods The eutopic endometrial cells were isolated,cultured and identified in vitro,then added to 10 ng/ml,50 ng/ml,100 ng/ml NGF.The growth and proliferation of the stromal cells were measured by MTT assay;Westen-blotting was used to measure the changed ERα.Results 50 ng/mlNGF could accelerate the proliferation of eutopic endometrial stromal cells of adenomyosis uterine,it showed a significant difference compared to other group(P<0.05);The expression of ERα in eutopic endometrial stromal cells were significantly increased after the treatment of 50 ng/ml NGF(P<0.01).Conclusion 50 ng/ml NGF can significantly accelerate the cell proliferation of eutopic endometrial stromal cells of adenomyosis uterine and increase the expression of ERα. |
| Key words: Nerve growth factor Adenomyosisi Estrogen receptor |
