| 摘要: |
| ［摘要］ 目的 评价联合应用微柱凝胶法和甘氨酸/EDTA放散试验进行诊断母婴血型不合新生儿溶血病（HDN）的临床价值。方法 对355例疑为母婴血型不合HDN的黄疸患儿血标本联合应用微柱凝胶法和甘氨酸/EDTA放散试验检测。结果 确诊为母婴血型不合HDN 277例（HDN的阳性检出率为78.03%），其中Rh-HDN 2例（0.72%），ABO-HDN 275例（99.28%）。ABO-HDN 275例中，母婴血型为O/A的占48.73%，O/B的占51.27%，B/AB和A/AB组合均为0例。在出生时间2 h≤n≤3 d、3 d<n≤5 d、5 d<n≤7 d、7 d<n≤13 d的黄疸儿中，HDN检出率分别为86.10%、73.02%、31.58%、14.29%。结论 联合应用微柱凝胶法和甘氨酸/EDTA放散试验检测HDN，能够准确、及时地为临床诊治提供依据，值得推广。 |
| 关键词: 母婴血型不合 新生儿溶血病 微柱凝胶法 甘氨酸/EDTA放散试验 高胆红素血症 |
| DOI：10.3969/j.issn.1674-3806.2015.04.05 |
| 分类号:R 722.18 |
| 基金项目:广西卫计委科研课题(编号：Z2012304） |
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| Combined application of micro-gel method and glycine/EDTA diffuse method in the detection of hemolytic disease of the newborn: report of 277 cases |
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LI Hai-lan, JIAO Wei, LI Ying-lan, et al.
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Department of Transfusion, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the clinical value of combined application of micro-gel method and glycine/EDTA diffuse method in the diagnosis of hemolytic disease of the newborn(HDN) caused by maternal-fetal blood type incompatibility.Methods Micro-gel method and glycine/EDTA diffuse method were jointly used to detect 355 blood samples from newborn with suspected HDN caused by maternal-fetal blood type incompatibility.Results HDN caused by maternal-fetal blood type incompatibility was diagnosed in 277 cases, the positive rate was 78.03%, of which Rh-HDN was detected in 2 cases(0.72%), ABO-HDN in 275 cases(99.28%). In 275 cases of ABO-HDN, mother and baby were both blood type O/A accounted for 48.73%, O/B accounted for 51.27%, no B/AB and A/AB blood type. For these newborn with jaundice between 2 h≤n≤3 d,3 d<n ≤5 d,5 d<n ≤7 d,7 d<n≤13 d after birth. HDN positive detection rates were 86.10%, 73.02%, 31.58%, 14.29%, respectively.Conclusion Joint application of micro-gel method and glycine / EDTA diffuse method in the detection of HDN, can accurately and timely provide the basis for clinical diagnosis and treatment. |
| Key words: Maternal-fetal blood type incompatibility Hemolytic disease of the newborn Micro-gel method Glycine/EDTA diffuse method Hyperbilirubinemia |
