| 摘要: |
| ［摘要］ 目的 探讨体外培养神经元在缺氧时细胞红蛋白（cytoglobin，Cygb）基因表达的变化规律。方法 运用无血清培养技术进行胎鼠皮质神经元培养6 d，用免疫组织化学方法进行纯度鉴定，随机分为4组，其中3个实验组分别置于0.5%～0.9% O2中8、16、24 h，对照组置于正常环境。用CCK8试剂盒测试细胞生存率，用Real-time PCR方法检测不同时点Cygb基因表达的情况。结果 神经元纯度鉴定纯度>99%；缺氧后神经元Cygb基因的表达随时间增长而递增。结论 体外培养神经元缺氧时Cygb基因表达增强，提示Cygb可能在神经细胞缺氧时发挥重要作用。 |
| 关键词: 细胞红蛋白 神经元 基因表达 缺氧缺血性脑损伤 |
| DOI：10.3969/j.issn.1674-3806.2016.01.03 |
| 分类号:R 363 |
| 基金项目:汕头市医疗重点科技计划项目（编号：汕府科2012-113号） |
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| Expression of cytoglobin gene in hypoxic neurons in vitro |
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YANG Han-hua, MA Hai-ran, XIAO Dan-ping, et al.
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Department of Pediatric, the Second Affiliated Hospital of Shantou University Medical College, Guangdong 515041, China
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| Abstract: |
| ［Abstract］ Objective To investigate the expression and changes of cytoglobin（Cygb） gene on hypoxic neurons in vitro.Methods The primary cortical neurons from newborn rats were cultured in a serum-free culture system. The neurons were identified by polyclonal antibody against neuron specific enolase on the 6th day and were randomly divided into 4 groups for assessing cell viability by CCK8 and for observing the gene expression of Cygb by Real-time PCR at 0 h, 8 h, 16 h, 24 h after hypoxic injury was induced with the concentration of oxygen between 0.5% and 0.9%.Results The purity of neurons was more than 99%; Expression of RNA Cygb was increased in a time-dependent manner, and there was significant difference in each group(P<0.05).Conclusion The expression of Cygb in neurons increases rapidly after hypoxic injury indicating that Cygb may have an important function in the process of protecting hypoxic neuron injury. |
| Key words: Cytoglobin Neurons Gene expression Hypoxic-ischemic encephalopathy |
