| 摘要: |
| ［摘要］ 目的 观察高血压左心室肥厚（LVH）人群中血浆miR-30a与LVH的关系，研究血浆miR-30a是否能作为高血压LVH患者临床诊断的标志物。方法 随机入选住院高血压患者中73例LVH患者和77例非LVH患者。行real-time PCR法检测血浆hsa-miR-30a的含量。用ROC曲线和Pearson相关性分析评价血浆hsa-miR-30a诊断高血压LVH的价值。结果 LVH组患者血浆hsa-miR-30a水平升高，患者血浆hsa-miR-30a水平的曲线下面积（AUC）为0.932，血浆hsa-miR-30a水平用于诊断高血压LVH有意义。确定hsa-miR-30a判断高血压LVH的最佳临界点为7.267（2-Δct×104），敏感度和特异度分别为94.5%、89.6%。Pearson相关性分析表明，患者血浆hsa-miR-30a水平与室间隔厚度（IVSd）和左室后壁厚度（LVPWd）均呈正相关（r值分别为0.636和0.637，P值均为0.000）。结论 高血压LVH患者血浆hsa-miR-30a升高。血浆hsa-miR-30a水平与IVSd和LVPWd呈正相关，血浆hsa-miR-30a水平用于诊断高血压LVH有意义。 |
| 关键词: 左心室肥厚 高血压 miR-30a |
| DOI：10.3969/j.issn.1674-3806.2016.07.03 |
| 分类号:R 541.6 |
| 基金项目:广东省医学科研基金立项课题（编号：A2014712） |
|
| Plasma hsa-miR-30a expression on hypertension patients with left ventricular hypertrophy |
|
PAN Wei, ZHAO Juan, YANG Ze-fu, et al.
|
|
Department of Cardiovascular Disease, Nanhai Hospital Affiliated to Southern Medical University, Guangdong 528200, China
|
| Abstract: |
| ［Abstract］ Objective To observe the correlation between plasma hsa-miR-30a and left ventricular hypertrophy（LVH） in hypertension patients and to study whether plasma hsa-miR-30a can be a diagnostic marker for patients with LVH or not.Methods Seventy-three cases with LVH were collected from the hypertensive patients as LVH group and 77 cases without LVH as the control group. The mRNA expression of hsa-miR-30a in the derived plasma was analyzed by real-time PCR. Evaluation of the sensitivity and specificity of hsa-miR-30a on the diagnosis of LVH was performed by analyzing the ROC curve. The association between plasma hsa-miR-30a level and ventricular wall thickness was assessed by Pearson correlation analysis.Results Compared with that in the control group, the level of hsa-miR-30a expression in LVH group was significantly higher［（5.16±0.31）（2-Δct×104）vs （14.28±0.92）（2-Δct×104）, P=0.000］. A receiver operating characteristic(ROC) curve showed that the areas under the curve(AUC) for the plasma level of hsa-miR-30a were 0.932. The optimal hsa-miR-30a cut-off value on receiver operating characteristic curve was 7.267（2-Δct×104）. The corresponding cut-off points with sensitivity and specificity were 94.5% and 89.6%, respectively. Pearson correlation analysis indicated that the level of hsa-miR-30a expression in plasma was positively associated with IVSd and LVPWd )(r=0.636 and 0.637, P=0.000 and 0.000, respectively).Conclusion Plasma hsa-miR-30a is elevated in hypertension patients with LVH and positively associated with left ventricular wall thickness. Elevated plasma hsa-miR-30a level is significant for the diagnosis of LVH in hypertension patients. |
| Key words: Left ventricular hypertrophy Hypertension MicroRNA-30a |
