| 摘要: |
| ［摘要］ 目的 探讨实时荧光定量聚合酶链反应（PCR）方法监测造血干细胞移植（HSCT）患者人巨细胞病毒（HCMV）感染的临床意义。方法 应用实时荧光定量PCR方法动态监测32例造血干细胞移植患者血标本HCMV-DNA载量，并与30名健康对照者进行对比分析。结果 32例患者阳性率为21.9%(7/32)，病死率为3.13%(1/32)。30名对照者阳性率为3.33%(1/30)。7例阳性患者均经CMV抗病毒治疗,其中1例在第56天死亡,1例在监测到70 d持续阳性，其余5例转阴。结论 实时荧光定量PCR方法检测HCMV-DNA对造血干细胞移植患者人巨细胞病毒感染的早期诊断及动态监测具有重要的临床意义。 |
| 关键词: 人巨细胞病毒 造血干细胞移植 实时荧光定量PCR |
| DOI：10.3969/j.issn.1674-3806.2016.01.19 |
| 分类号:R 63 |
| 基金项目: |
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| Clinical significance of monitoring human cytomegalic inclusion disease virus after haemopoietic stem cell transplantation by real-time PCR |
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XIAO Xiao-hong, CHEN Wei
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Department of Blood Transfusion, the Central Hospital of Loudi, Hunan 417000, China
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| Abstract: |
| ［Abstract］ Objective To evaluate the value of real-time fluorogenic quantitative PCR assay for early detecting and dynamic monitoring human cytomegalovirus(HCMV) activation after hematopoietic stem cell transplantation(HSCT).Methods HCMV-DNAs of 32 bonemarrow transplant recipients and 30 healthy people were dynamically monitored using real-time PCR.Results Among the 32 patients, the positive rate was 21.9%(7/32) and the case fatality ratio was 3.33%(1/30). In the control group, the positive rate was 3.13%(1/32). Among all 7 positive patients who were treated with antiviral therapy, One patient died in 56 days and the other 6 patients was negative except that one patient was positive for 70 days.Conclusion The quantitative detection for HCMV-DNA by real-time PCR has clinical significance for early diagnosing and dynamically monitoring HCMV in fections in HSCT patients. |
| Key words: Human cytomegalovirus(HCMV) Hematopoietic stem cell transplantation(HSCT) Real-time PCR |
